The Linkage Map Construction and QTL Analysis of Growing Related Traits in Salix matsudana Using SLAF-seq Method
Willow (Salix) is one of the most important ornamental tree species in land. They are widely distributed and have the advantages of salt-resistant, fast growing, and good material, thus mainly used for bioenergy, afforestation, soil and water conservation. To date, the molecular genetics of Salix are less studied. In this paper, an intraspecific F1 hybrid population of Salix matsudana was established by crossing the salt-sensitive “Yanjiang” variety (as the female parent) with the salt-tolerant “9901” variety (as the male parent), and high resolution genetic map was built for S. matsudana using SLAF-seq method. 10 fast-growing quantitative traits of the S. matsudana mapping population such as the sprout length, root number, the longest root, total root length, root fresh weight, root dried weight, leaf length, leaf width, leaf fresh weight, and leaf dried weight were relatively significant segregation. QTLs analysis of these fast-growing related traits was conducted using Map QTL5.0 software by the Internal Mapping method. A total of 170 QTLs were identified and each QTL could explain 10.0-16.4% of the corresponding phenotypic variation, respectively. QTL mapping of growing related traits could not only provide theoretical basis for key gene cloning that controlling these traits, but also laid the practical foundation for marker assisted breeding of S. matsudana.
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