Hyper Production of Catalase by Bacillus subtilis GCU-4 Mutant Strain and Optimization of Fermentation Process using Response Surface Methodology
Green chemistry technologies approaches to preventing the environmental pollution, using renewable feedstocks and energy sources. Environment friendly waste management has become the global priority. The aim of present investigation was to improve the catalase yield by treating the parent Bacillus subtilis BS-21 strain with UV irradiations using agroindustrial waste (corn cob) in submerged fermentation. Seven positive mutants were isolated on hydrogen peroxide containing agar plates. GCU-4 mutant strain showed 775.84 U/mL catalase activity higher than parent strain BS-21 (397.54U/ml) in optimized fermentation medium. The effect of main variables i.e. pH (8.25), temperature (35°C), inoculums age (42 hrs) and fermentation time (60 hrs) on the production of catalase was evaluated using response surface methodology with a full factorial central composite design. A second order quadric model and response surface method show that mutual interaction between these variables resulting to increase 2.68 fold catalase activity as compared to non optimized environmental condition. The analysis of variance showed a satisfactory adjustment of the quadric model with the experimental data. It was concluded from the study, strain improvement and response surface methodology (RSM) techniques maximize the catalase yield by optimization the operational factors.
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