Xanthan gum biochemical profiling, antioxidant, antibacterial, biofilm inhibition and mutagenic potential
Xanthan is a useful natural exo-polymer. The aim of study was biochemical characterization of xanthan. Carboxymethylation grafting of the gum was performed to modify it. Gum samples were characterized by using thermogravimetric analysis (TGA), scanning electron microscopy (SEM), laser-induced breakdown spectroscopy (LIBS), high Performance liquid chromatography (HPLC) and fourier transform infrared spectroscopy (FTIR). For its biochemical profile enzyme activity, antioxidant, antimicrobial, biofilm inhibition and mutagenic behavior were studied. LIBS analysis showed presence of different elements. HPLC analysis indicated that monosaccharides of xanthan were glucose and mannose. SEM reveals that xanthan gum has fibrous structure. FTIR analysis indicated presence of different functional groups. TGA told about temperature effect, as the gum become thermally more stable after modification. Xanthan gum in its crude, purified and modified form has shown antioxidant activity by scavenging DPPH radicals and total phenolic content presence was also confirmed because of its antioxidant activity. The antibacterial potential of gum sample was enhanced after the modification. Biofilm inhibition was noticed by all xanthan samples. Biofilm inhibition was also high of modified xanthan as compared to crude and purified. Xanthan proves to be non-toxic. Mutagenic study showed no toxicity indicating non-mutagenicity. So, xanthan has its nutritional, antioxidant, antibacterial and biofilm inhibitor uses in food and pharmaceutical industry.
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