Schisandrin B-loaded polymeric micelles for protecting SH-SY5Y cells from H2O2-induced oxidative stress
Alzheimer’s disease (AD) is a worldwide aged disease which is closely related to the abnormal accumulation of amyloid β (Aβ) caused by oxidative stress. Traditional Chinese medicine active ingredient Schisandrin B (Sch B) has been reported as an effective antioxidant by scavenging reactive oxygen species (ROS). Due to the poor solubility and low transmission efficiency of Sch B across blood–brain barrier (BBB), Sch B-loaded polymeric micelles (SB-PMs) were developed based on amphiphilic block copolymers, methoxy poly(ethylene glycol)-poly(lactide) (mPEG-PLA), using the emulsion solvent evaporation method. The encapsulation efficiency and drug loading of Sch B were 81.75%±1.07% and 16.35%±0.21%, respectively. Sch B was constantly released from SB-PMs for more than 48 hours, with leveling off after 12 h, and cumulative release of Sch B was approximately 70%. Their cellular uptake by SH-SY5Y cells increased significantly and was highly time-dependent. Then, the protective effect of SB-PMs on the viability and antioxidant ability of SH-SY5Y cells were investigated. It was found that the SB-PMs suppressed the cytotoxicity and ROS generation of H2O2-induced SH-SY5Y cells more obviously than free Sch B, especially at the equivalent concentration of 5 μg/mL Sch B, 12h pre-incubation. In light of this work, further in vivo investigations are needed to make SB-PMs a promising therapeutic tool for H2O2-induced oxidative stress.
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